Journal: Pharmaceuticals

Article Title: Development of a New Radiofluorinated Quinoline Analog for PET Imaging of Phosphodiesterase 5 (PDE5) in Brain

doi: 10.3390/ph9020022

Figure Lengend Snippet: ( A ) Semi-preparative radio- and UV-HPLC chromatograms of [ 18 F] ICF24027 (conditions: Reprosil-Pur C18-AQ, 250 mm × 10 mm, 62% ACN/20 mM NH 4 OAc aq. , 3.0 mL/min). ( B ) Analytical radio- and UV-HPLC chromatograms of the final product of [ 18 F] ICF24027 spiked with the nonradioactive reference ICF24027 (conditions: Reprosil-Pur C18-AQ, 250 mm × 4.6 mm, gradient with eluent mixture of ACN/20 mM NH 4 OAc aq., 1.0 mL/min).

Article Snippet: Semi-preparative HPLC separations were performed on a JASCO LC-2000 system, incorporating a PU-2080-20 pump, an UV/VIS-2075 detector coupled with a gamma radioactivity HPLC detector whose measurement geometry was slightly modified (Gabi Star, raytest Isotopenmessgeräte GmbH) and a fraction collector (CHF-122SC, Advantec, Dublin, CA, USA).

Techniques:

Journal: Biosensors

Article Title: Radiometal-Based PET/MRI Contrast Agents for Sensing Tumor Extracellular pH

doi: 10.3390/bios12020134

Figure Lengend Snippet: Stability and metabolism of the 68 Ga PET co-agent. ( a ) RadioHPLC of the 68 Ga PET co-agent (blue trace) was incubated in human serum (green trace) and PBS (orange trace) for one hour at 37 °C and showed complete stability, with no presence of free 68 Ga (red trace). ( b ) RadioHPLC of the 68 Ga PET co-agent from urine collected 20 min post-injection from the mouse imaged with simultaneous PET/MRI, as shown in . The results showed the presence of two new species (blue trace) that were different than intact 68 Ga PET co-agent (green trace) or free 68 Ga (orange trace).

Article Snippet: RadioHPLC was performed on an analytical Agilent 1260 Infinity II Series (Agilent Technologies, Santa Clara, CA, USA) with a XBridge C18 3.5 μm column with 4.6 × 250 mm dimensions using a Flow-RAM radioHPLC detector (LabLogic Systems Ltd., Brandon, FL, USA), for compound identification and quality control.

Techniques: Incubation, Injection

Journal: RSC Advances

Article Title: Quassinoids from Malaysian Eurycoma longifolia significantly increased the expression of the melatonin biosynthesis-related enzyme gene (AANAT) †

doi: 10.1039/d5ra01703c

Figure Lengend Snippet: (a) HPLC analysis result of three quassinoids (1–3); (b) HRMS spectra of the three quassinoids (1–3).

Article Snippet: The Fr.3 (77.3 mg) was separated by semi-preparative HPLC (GL Sciences Inc. ∅ 20 × 250 mm, 5 μm particles; UV detector 205 nm, flow rate: 8 mL min −1 ) with MeCN/H 2 O gradient (20 : 80 → 100 : 0) as a mobile phase to yield to 3 (21.3 mg, t R = 30 min).

Techniques: